Table 2.
Compar ison of flavanol and phenolic acid contents of nonfermented and fermented juices of Elaeagnus multiflora fruits
Physicochemical characteristics1) | Thermal processing conditions |
Control | 80°C/30 min | 100°C/30 min | 120°C/30 min |
NFJEM2) | FJEM3) | NFJEM | Wine | NFJEM | FJEM | NFJEM | FJEM |
Flavanol | | | | | | | | |
Catechin | 102.44±4.01b | 68.77±3.43c | 109.12±5.02b | 72.57±3.27c | 124.56±5.42ab | 81.20±3.24bc | 147.88±5.91a | 99.45±3.56b |
Catechin gallate | 40.23±1.61a | 32.11±1.44ab | 38.52±1.63a | 29.80 | 32.14±1.45ab | 24.01±1.20b | 28.12±1.40ab | 20.11±1.14b |
Epicatechin | 203.11±9.98cd | 154.22±7.71d | 234.14±11.70b | 176.33±8.01d | 278.53±11.92ab | 226.12±11.55b | 311.35±15.56a | 264.55±11.88ab |
Epicatechin gallate | 67.23±3.36a | 37.20±1.67b | 60.38±3.02a | 30.24±1.51c | 47.52±1.92ab | 21.33±1.05d | 40.27±1.61b | 17.26±0.86d |
Epigallocatechin | 87.33±4.36b | 42.57±1.92b | 101.26±4.56b | 55.41±2.77cd | 113.89±5.69ab | 69.87±3.49c | 132.29±5.91a | 82.19±4.11b |
Epigallocatechin gallate | 15.61±0.78a | 8.55±0.42b | 15.89±0.82a | 8.76±0.44b | 15.66±0.77a | 8.98±0.40b | 16.02±0.80a | 8.81±0.39b |
Total | 500.34 | 334.87 | 543.42 | 364.35 | 596.64 | 422.53 | 659.91 | 483.56 |
Phenolic acids | | | | | | | | |
Caffeic acid | 2.57±0.12a | 2.41±0.11ab | 2.51±0.13a | 2.36±0.10ab | 2.18±0.10b | 2.20±0.10b | 1.97±0.08b | 1.46±0.06c |
t-Cinnamic acid | 1.37±0.06a | 0.83±0.04c | 1.32±0.05a | 0.82±0.03c | 1.19±0.05ab | 0.98±0.05b | 1.01±0.06b | 0.74±0.02c |
p-Coumaric acid | 8.11±0.41a | 5.74±0.28b | 7.89±0.39a | 5.43±0.22b | 7.56±0.35a | 4.99±0.20b | 7.16±0.37a | 4.03±0.18b |
Ferulic acid | nd | nd | 0.12 | nd | 5.78±0.28a | 2.32±0.12c | 11.21±0.56a | 5.02±0.25a |
Gallic acid | 7.89±0.39c | 6.67±0.33de | 8.06±0.40c | 6.99±0.35d | 8.58±0.43d | 7.11±0.35c | 9.87±0.49a | 6.44±0.32e |
p-hydroxylbenzoic acid | 2.86±0.14d | 3.41±0.17c | 3.42±0.16c | 4.21±0.21b | 3.87±0.19bc | 5.88±0.29a | 5.66±0.24a | 5.92±0.30a |
Protocatechuic acid | 13.66±0.68bc | 12.03±0.60c | 14.02±0.70b | 12.57±0.63c | 14.89±0.74b | 13.21±0.66bc | 18.43±0.92a | 10.22±0.51d |
Tannic acid | 1.58±0.08a | nd4) | 0.53±0.03b | nd | nd | nd | nd | nd |
Salicylic acid | 43.60±2.18d | 16.77±0.83e | 52.36±2.61c | 22.44±1.12e | 73.81±3.69b | 43.06±2.15d | 101.24±5.00a | 60.53±3.02bc |
Vanillic acid | 9.77±0.48b | 5.46±0.27d | 11.24±0.56b | 7.86±0.39c | 13.02±0.65ab | 9.87±0.49b | 15.99±0.79a | 10.11±0.50b |
Total | 81.64 | 51.86 | 90.23 | 54.82 | 117.86 | 79.75 | 156.55 | 94.36 |
All data are presented as the mean SD of triplicate determinations. Means with different lowercase letters (a, b, c, d, and e) indicate significant differences of fermentation times by Tukey s multiple range test (p<0.05).
)NFJEM, nonfermented juice of Elaeagnus multiflora, Elaeagnus multiflora juices were contained 0.5% (w/v) of (NH)4HPO4 and 24 °Brix of sugar.
FJEM, fermented juice of Elaeagnus multiflora, S. cerevisiase CS02 were innoculated into Elaeagnus multiflora juice containing 0.5% (w/v) of (NH)4HPO4 and 24 °Brix of sugar and there were fermented at 25°C for 9 days.
nd, not detected.