Table 3.
Enzyme activities of mold separated from traditional meju and koji

Bacteria No. α-Amylase (unit/g)3) β-Amylase (unit/g)4) Acidic protease (unit/g)5) Neutral protease (unit/g)6)
1-M-11) 979.02 2851.95 441.50 372.28
2-M-11) 1053.98 755.82 10< 29.71
4-M-11) 1847.48 4950.77 21.42 84.30
6-M-11) 1942.56 6324.20 326.97 296.47
8-M-11) 988.42 2467.98 195.63 298.22
9-M-11) 183.00 913.98 42.81 143.33
10-M-11) 1288.48 6220.34 1020.75 1573.20
11-M-11) 70.39 2244.77 713.28 768.37
12-M-11) 1912.55 6564.33 279.03 361.12
13-M-11) 551.53 543.12 194.63 156.50
14-M-11) 234.29 1337.09 712.04 651.98
15-M-11) 464.45 1714.85 440.34 638.47
16-M-11) 1845.16 2317.53 218.13 269.48
17-M-11) 922.91 2302.39 331.16 585.65
18-M-11) 1150.39 2276.10 185.82 890.84
19-M-11) 47.97 284.08 10< 55.80
20-M-11) 109.93 317.79 10< 52.76
21-M-11) 299.93 193.47 10< 35.38
24-M-11) 34.54 200.49 42.90 61.55
26-M-11) 451.99 1118.55 243.77 340.40
27-M-11) 533.99 1145.04 10< 61.32
28-M-11) 335.21 538.64 73.46 135.86
30-M-11) 1966.54 6856.87 41.48 189.82
31-M-11) 121.42 439.71 100.98 108.97
32-M-11) 206.22 409.34 96.45 110.02
33-M-11) 227.45 305.62 25.62 94.73
36-M-11) 98.76 140.96 38.27 59.06
37-M-11) 70.28 221.92 50.76 75.89
38-M-11) 162.85 268.45 10< 96.67
39-M-11) 1486.69 3751.83 32.25 68.64
40-M-11) 435.61 969.50 294.54 523.54
41-M-11) 15.56 389.99 114.48 138.02
1-K-M-12) 221.88 492.44 41.77 80.58
2-K-M-12) 1815.31 6283.04 115.32 139.49
3-K-M-12) 1486.69 3751.83 32.35 68.64
4-K-M-12) 883.56 2810.08 250.88 304.03
Bacteria separated from traditional Meju.
Bacteria separated from Koji.
One unit of α-amylase was the amount of enzyme which released reducing sugar from 1 mg soluble starch for 10 minutes at 25°C and pH 5.9 under specific conditions.
One unit of β-amylase was the amount of enzyme which released maltose from 1 mg soluble starch for 10 minutes at 25°C and pH 5.9 under specific conditions.
One unit of acidic protease was the amount of enzyme which released 1 µg tyrosine from 1 mg casein for 10 minutes at 30°C and pH 3.0 under specific conditions.
One unit of neutral protease was the amount of enzyme which released I µg tyrosine from 1 mg casein for 10 minutes at 30°C and pH 6.0 under specific conditions.