Table 7.
Effect of vitamin C on Immunohistochemical staining1) in rats liver

No.	dilution	area	1	2	3	4
Group	dilution	area	1	2	3	4
1	1:100	CV	–	–	–	–

		PA	–	–	–	–

	1:200	CV	–	–	–	–

		PA	–	–	–	–

2	1:100	CV	++	+	++	++

		PA	+	–	–	+

	1:200	CV	++	++	++	++

		PA	–	+	–	+

3	1:100	CV		++	++	+

		PA		–	–	–

	1:200	CV	+	+	+	++

		PA	–	–	–	–

4	1:100	CV	++	++	++	+

		PA	–	–	–	–

	1:200	CV	++	++	++	+

		PA	–	–	–	–

5	1:100	CV	++	+	+	+

		PA	+	–	–	–
	1:200	CV	+	+	+	+
		PA	–	–	–	–

Immunohistochemical staining of AFB₁observed by two observers were represented at (–), (+), (++), (+++). (–): negative reaction, (+): weak stainability, (++): medium stainability, (+++): strong stainability, CV: central vein, PV: portal vein. G1: control group (no treated group), G2: AFB₁–treated group, G3: the group treated with AFB1 and vitamin C, G4: the group treated with X–ray and AFB1, G5: the group treated with X–ray and AFB1 with vitamin C.