Instrument | Conditions | ||
---|---|---|---|
Column | SunfireTM C18 5.0 μm, 4.6 mm×250 mm | ||
Column temp. | 40℃ | ||
Mobile phase (Gradient) | Time (min) | Solvent A1) | Solvent B2) |
0 | 8 | 92 | |
23 | 8 | 92 | |
26 | 15 | 85 | |
36 | 30 | 70 | |
40 | 45 | 55 | |
43 | 45 | 55 | |
45 | 8 | 92 | |
53 | 8 | 92 | |
Detector | Waters 996 Photodiode Array Detector (280 nm) | ||
Flow rate | 1.0 mL/min | ||
Injection volume | 10 μL | ||
Run time | 53 min |