Table 1
HPLC conditions for the analysis of phenolic compounds

Instrument Conditions
Column SunfireTM C18 5.0 μm, 4.6 mm×250 mm
Column temp. 40℃
Mobile phase (Gradient) Time (min) Solvent A1) Solvent B2)
0 8 92
23 8 92
26 15 85
36 30 70
40 45 55
43 45 55
45 8 92
53 8 92
Detector Waters 996 Photodiode Array Detector (280 nm)
Flow rate 1.0 mL/min
Injection volume 10 μL
Run time 53 min
Solvent A, Acetonitrile.
Solvent B, 0.5% H3PO4-Water.