Table 1
Total phenolic contents and radical scavenging activity of Geranium thunbergii solvent-partitioned fractions
| TPC2) (mg/g) | DPPH radical scavenging activity (%, μg/mL) | ABTS radical scavenging activity (%, μg/mL) |
25 | 50 | 100 | 200 | 25 | 50 | 100 | 200 |
HF1) | 83.72±5.044)E5) | 7.24±1.01Ec6) | 13.43±0.67Ec | 25.03±0.67Db | 45.41±1.57Ea | 0.50±1.09Ed | 6.63±0.33Gc | 19.65±1.36Db | 42.76±0.91Da |
CF | 148.83±1.40C | 12.60±0.67Dd | 26.24±1.01Dc | 42.27±1.07Cb | 71.93±1.83Da | 6.82±1.28Ed | 21.36±1.90Ec | 38.16±2.71Bb | 74.33±1.22Ba |
EF | 604.28±1.95A | 54.64±0.35Bc | 80.88±1.34Bb | 93.31±0.25Aa | 93.98±0.10Ba | 47.14±1.58Bc | 80.12±2.41Cb | 99.50±0.17Aa | 99.99≥Aa |
BF | 465.65±4.88B | 43.70±0.10Cc | 73.48±1.15Cb | 92.21±0.60Aa | 92.76±0.10Ba | 38.53±0.50Cd | 70.72±1.28Dc | 97.37±1.14Ab | 99.99≥Aa |
WF | 98.52±1.18D | 8.78±3.37DEc | 13.76±3.80Ec | 28.01±2.64Db | 45.80±3.48Ea | 10.43±7.19Dc | 15.54±6.58Fc | 33.66±5.68Cb | 57.69±5.10Ca |
BHA3) | | 9.94±3.39DEd | 27.02±3.57Dc | 64.86±2.23Bb | 87.85±0.83Ca | 54.87±4.02Ac | 87.70±2.94Bb | 99.99≥Aa | 99.99≥Aa |
AA3) | | 76.85±3.68Ab | 96.52±0.29Aa | 96.57±0.25Aa | 96.91±0.25Aa | 50.84±4.51ABb | 99.05±0.92Aa | 99.99≥Aa | 99.99≥Aa |
HF, n-hexane fraction; CF, chloroform fraction; EF, ethyl acetate fraction; BF, butanol fraction; WF, water fraction; BHA, butylated hydroxyl anisole; AA, ascorbic acid.
TPC was determined with reference to the standard curve of gallic acid content of samples using spectrophotometer.
Positive controls were used butylated hydroxyl anisole and ascorbic acid.
Data represent the means SD of 3 determinations.
A-GMeans of the different superscripts within same column are significantly different at p<0.05.
a-dMeans of the different superscripts within same row are significantly different at p<0.05.