Table 2. Result of method validation for the derivatized monosaccharides
| Peak# | Monosaccharide | Calibrationcurve | Correlation coefficient(r2) | Precision1)(% RSD) | Accuracy2)(%) | LOD(ug/mL) | LOQ(ug/mL) |
| 1 | Mannose | y=24011x-1143 | 0.99996 | 0.52 | 98.45-108.21 | 2.13 | 6.46 |
| 2 | Rhamnose | y=23929x-27180 | 0.99996 | 0.42 | 97.32-101.31 | 1.60 | 4.85 |
| 3 | Glucuronic acid | y=21409x+99533 | 0.99991 | 0.36 | 89.21-97.87 | 1.03 | 3.13 |
| 4 | Glucose | y=21549x+10714 | 0.99994 | 0.18 | 92.76-103.56 | 1.03 | 3.11 |
| 5 | Galactose | y=24412x-11303 | 0.99999 | 0.69 | 98.56-106.21 | 1.14 | 3.46 |
| 6 | Xylose | y=30281x-35587 | 0.99999 | 0.29 | 94.53-109.76 | 1.14 | 3.44 |
| 7 | Fucose | y=23932x-12315 | 0.99995 | 0.30 | 97.11-105.21 | 0.76 | 2.31 |
1)Precision was evaluated using five replicates performed on a intra-day.
2)Accuracy was measured of the closeness of the analytical result of the true value determined by analyzing a spiked sample.