Table 3. HPLC conditions for analysis of vitamin C

Instrument Agilent HPLC
Solvent Acetonitrile:50nM NH4H2PO4 = 70:30 (v/v)
Flow rate 1.0 mL/min
Wavelength 254 nm
Column YMC-Pack Polyamine II (4.6×250 mm, 5 μm, Japan)
Oven temperature 40°C
Injection volume 20 μL