Parameters | Condition |
---|---|
HPLC system | Waters 2695 |
Column | Agilent ZORABX Eclipse XDB-C18 (5 μm, 4.6×250 mm, Santa Clara, CA, USA) |
Detector | UV 300 nm |
Flow rate | 1.0 mL/min |
Column temperature | 35°C |
Injection volume | 10 μL |
Mobile phase | Methanol:H2O (0.1% formic acid)=30:70 |