Table 3. Changes in free amino acid contents in mulberry juice and mulberry vinegarFree amino acid

Free amino acid (mg%)	Sample¹⁾		t-value
Free amino acid (mg%)	MJ	MV	t-value
Aspartic acid	18.29±0.03²⁾	0.55±0.04	597.677^***3)
Threonine	2.75±0.00	0.89±0.66	54.412^***
Serine	7.78±0.01	0.26±0.04	336.006^***
Asparagine	58.76±0.05	1.22±0.21	451.801^***
Glutamic acid	13.82±0.02	1.02±0.12	185.357^***
Sarcosine	1.03±0.02	0.48±0.25	3.751^NS
α-Aminoadipic acid	0.45±0.01	0.89±0.28	-2.713^NS
Glycine	1.38±0.00	0.42±0.08	20.035^***
Alanine	7.83±0.02	0.63±0.07	165.562^***
Citrulline	0.39±0.01	0.37±0.15	0.255^NS
α-Amino-n-butyricacid	0.21±0.01	0.21±0.05	-0.141^NS
Valine	2.86±0.03	0.81±0.07	46.518^***
Cystein	2.66±0.02	2.31±0.17	3.540^NS
Methionine	1.50±0.00	0.63±0.08	17.640^**
Cystathionine	0.27±0.01	0.26±0.06	0.221^NS
Isoleucine	2.62±0.02	1.22±0.10	24.015^**
Leucine	5.29±0.01	1.69±0.07	92.275^***
Tyrosine	5.20±0.02	2.36±0.12	41.684^***
Phenylalanine	3.71±0.02	2.99±0.12	10.280^**
β-Alanine	0.91±0.00	0.36±0.02	36.997^***
β-Aminoisobutyric acid	1.44±0.02	1.08±0.05	12.310^***
γ-Amino-n-butyric acid	16.73±0.01	0.30±0.02	1,242.097^***
2-Aminoethanol	0.96±0.01	-⁴⁾	-
Ammonium chloride	1.45±0.00	0.04±0.00	460.085^***
Hydroxylysine	0.09±0.00	-	-
Ornithine	0.27±0.00	0.96±0.00	-189.639^***
Lysine	3.21±0.01	4.48±0.01	-176.074^***
1-Methylhistidine	-	0.02±0.00	-
Histidine	1.65±0.00	1.14±0.01	129.060^***
3-Methylhistidine	-	0.04±0.00	-
Anserine	2.45±0.01	2.16±0.01	64.156^***
Carnosine	0.22±0.10	0.33±0.01	-1.914^NS
Arginine	6.68±0.11	4.41±0.09	27.917^***
Total	172.95±0.08	34.52±2.18

MJ, mulberry juice; MV, mulberry vinegar.
Values are mean±SD.
Significant difference by the Student’s t-test (^**p<0.01, ^***p<0.001, ^NSnot significant).
Not detected.