Table 1. HPLC conditions for analysis of sialic acid
Conditions
Column
Phenomenex Luna C
18
(2) 100 Å (250 × 4.6 mm, 5 μm)
Eluent
AcOH/MeOH/H
2
O = 0.05/25/75 (isocratic mode)
Flow rate
1.2 mL/min
Oven Temp.
30°C
Detector
Fluorescence ex. 373 nm, em. 448 nm
Run time
30 min