Table 2. Accuracy of immunoaffinity-HPLC/PDA for vitamin B₇ and B₁₂ analyses

Analytes	Samples1)	Reference value (μg/ 100 g)2)	Analysis values (μg/ 100 g)3)	Recovery (%)4)
Vitamin B7 (biotin)	SRM 1549a	15.20±1.60	15.23±0.89	100.2
	SRM 3290	142.00±23.00	140.61±2.63	99.0
Vitamin B12 (cyanocobalmin)	SRM 1849a	4.82±0.85	4.85±0.07	100.6
	SRM 3290	9.50±2.50	9.63±0.17	101.3

SRM (standard reference material) 1549a, 1849a and 3290 indicate whole milk powder, infant/adult nutritional formula and dry cat food, respectively.
The reference values for the contents of corresponding analytes in SRMs provided by NIST.
The analytical value obtained in this study.
Recovery (%)=100×(analysis value/reference value).