Table 2. Changes in free amino acid contents during fermentation of soy-powder milk by mixtures of probiotics L. brevis WCP02 and L. plantrum P1201

Contents (mg/100 g dw1))	Fermentation time (h)
	0	12	24	36	48	60	72
Non-essential amino acids
Glutamic acid	100.38±5.022)	234.88±11.74	290.13±14.51	271.86±13.59	236.79±11.84	182.48±9.12	177.91±8.91
γ-Aminobutyric acid	32.05±1.61	47.20±2.36	65.30±3.27	75.72±3.79	82.53±4.13	83.22±4.16	76.22±3.81
Aspartic acid	12.61±0.63	31.51±1.58	32.68±1.63	13.81±0.69	8.25±0.41	26.38±1.32	30.26±1.51
Serine	28.32±1.42	86.15±4.31	117.31±5.87	107.24±5.36	55.37±2.77	83.14±4.16	108.99±5.45
Aminoadipic acid	7.31±0.37	26.71±1.34	51.01±2.55	72.42±3.62	58.34±2.92	55.15±2.76	87.82±4.39
Glycine	15.08±0.75	61.98±3.11	79.11±3.96	77.08±3.85	40.25±2.01	57.33±2.87	75.29±3.76
Alanine	83.14±4.16	141.45±7.07	159.47±7.97	117.63±5.88	72.60±3.63	99.38±4.97	123.01±6.15
Cystathionine	5.31±0.27	8.90±0.45	8.51±0.43	8.02±0.41	4.71±0.24	7.64±0.38	10.88±0.54
Tyrosine	26.95±1.35	21.50±1.08	8.22±0.41	9.06±0.45	5.85±0.29	9.46±0.47	12.99±0.65
β-Alanine	32.43±1.62	73.58±3.68	87.06±4.35	80.70±4.04	49.59±2.48	33.19±1.66	37.99±1.91
Aminoethanol	4.92±0.25	6.22±0.31	7.71±0.39	6.90±0.35	5.29±0.26	7.53±0.38	7.35±0.37
Hydroxyproline	16.34±0.82	12.50±0.63	17.02±0.85	16.00±0.80	2.12±0.11	23.23±1.16	27.26±1.36
Ornithine	2.46±0.12	193.72±9.69	233.48±11.67	217.50±10.88	126.26±6.31	179.51±8.98	215.45±10.77
Anserine	6.88±0.34	10.37±0.52	9.93±0.50	11.84±0.59	5.09±0.25	13.78±0.69	14.33±0.72
Carnosine	ND3)	41.85±2.09	30.89±1.54	75.03±3.75	7.85±0.39	9.07±0.45	10.83±0.54
Arginine	153.13±7.66	ND	2.39±0.12	5.98±0.30	1.64±0.08	2.32±0.12	2.72±0.14
Total	527.31	998.523	1,200.22	1,166.79	762.53	872.81	1,019.30
Essential amino acids
Threonine	0.93±0.05	3.91±0.21	69.66±3.48	54.37±2.72	28.69±1.43	43.14±2.16	58.76±2.94
Valine	26.11±1.31	86.40±4.32	93.82±4.69	76.27±3.81	38.40±1.92	55.71±2.79	77.17±3.86
Methionine	14.40±0.72	27.74±1.39	30.53±1.53	24.19±1.21	12.78±0.64	18.91±0.95	26.34±1.32
Isoleucine	24.01±1.21	48.54±2.43	55.96±2.81	43.95±2.20	17.07±0.85	26.12±1.31	40.39±2.02
Leucine	49.19±2.46	139.14±6.96	181.81±9.09	161.10±8.06	69.49±3.47	98.96±4.95	144.33±7.22
Phenylalanine	44.93±2.25	120.31±6.02	162.21±8.11	137.90±6.90	69.04±3.45	93.18±4.66	128.90±6.45
Lysine	36.93±1.85	77.44±3.87	89.32±4.47	76.08±3.80	48.09±2.41	59.61±2.98	77.21±3.86
Histidine	14.63±0.73	29.72±1.49	39.21±1.96	36.14±1.81	23.29±1.16	29.54±1.48	36.00±1.81
Total	211.13	533.2	722.52	610.0	306.85	425.17	589.1
Total amino acids	738.44	1,531.72	1,922.74	1,776.79	1,069.38	1,297.98	1,608.4
Ammonia	19.61±0.98	34.84±1.74	42.19±2.11	40.48±2.02	26.69±1.33	25.84±1.29	48.61±2.43

dw, dry weight.
All values are represented as the mean±SD of triplicate determination.
ND, not detected.