Table 2. HPLC conditions of pectolinarin analysis for C. setidens Nakai extracts

Instrument Shimadzu HPLC system (HPLC-UV)
Column XBridge C18 (4.6×250 mm, 5 um)
Mobile phase Solution(A) : 0.1% TFA in D.W
Solution(B) : Acetonitrile
Time (min) B Conc. (%)
0.1 10
5 10
15 30
20 47
30 47
33 10
40
Oven temp. 35°C
Wavelength 330 nm
Injection volume 10 μL
Flow rate 1 mL/min
Run time 40 min