Instrument | Shimadzu HPLC system (HPLC-UV) | |
---|---|---|
Column | XBridge C18 (4.6×250 mm, 5 um) | |
Mobile phase | Solution(A) : 0.1% TFA in D.W | |
Solution(B) : Acetonitrile | ||
Time (min) | B Conc. (%) | |
0.1 | 10 | |
5 | 10 | |
15 | 30 | |
20 | 47 | |
30 | 47 | |
33 | 10 | |
40 | − | |
Oven temp. | 35°C | |
Wavelength | 330 nm | |
Injection volume | 10 μL | |
Flow rate | 1 mL/min | |
Run time | 40 min |