Parameters | Conditions | ||
---|---|---|---|
Injection volume | 10 μL | ||
Detector | DAD | ||
Wavelength | 254, 320 nm | ||
Column temperature | 30°C | ||
Flow rate | 1.0 mL/min | ||
Mobile phase | A: 1% formic acid in water, | ||
Gradient program | Time (min) | A (%) | B (%) |
0 | 95 | 5 | |
5 | 95 | 5 | |
10 | 90 | 10 | |
15 | 90 | 10 | |
20 | 80 | 20 | |
25 | 80 | 20 | |
30 | 70 | 30 | |
40 | 55 | 45 | |
50 | 40 | 60 | |
55 | 40 | 60 | |
60 | 5 | 95 | |
65 | 5 | 95 | |
70 | 95 | 5 | |
75 | 95 | 5 |