Table 1. Conditions of HPLC analysis for phenolic acid

Parameters Conditions
Injection volume 10 μL
Detector DAD
Wavelength 254, 320 nm
Column temperature 30°C
Flow rate 1.0 mL/min
Mobile phase A: 1% formic acid in water,B: Methanol
Gradient program Time (min) A (%) B (%)
0 95 5
5 95 5
10 90 10
15 90 10
20 80 20
25 80 20
30 70 30
40 55 45
50 40 60
55 40 60
60 5 95
65 5 95
70 95 5
75 95 5