Table 2. Conditions of HPLC analysis for gallic acid
Parameters
Conditions
Injection volume
5 μL
Detector
DAD
Wavelength
210 nm
Column temperature
40°C
Flow rate
0.8 mL/min
Mobile phase
A: 0.2% phosphoric acid in water
B: 0.2% phosphoric acid in acetonitrile
Gradient program
Time (min)
A (%)
B (%)
0
100
0
23
45
55
23.1
100
0
30
100
0