Table 2. Real-time PCR conditions

Gene	Real-time PCR condition
TNF-α, IL-1β, MCP-1, PTGES2, GAPDH	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, followed by each 95°C, 60°C, 95°C for 15 s
IL-6	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, 72°C for 15 s, followed by each 95°C, 60°C, 95°C for 15 s
β-Actin	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s or 95°C for 10 s, 60°C for 20 s, 72°C for 15 s, followed by each 95°C, 60°C, 95°C for 15 s