Table 1. Effects of solvents in Soxhlet and maceration extraction methods for 5 h with the solvent-to-solid ratio of 100:10
Method | Solvent | SP (g-BSAE/100 g DW) | TS (g-GE/100 g DW) | RS (g-GE/100 g DW) | TPC (mg-GAE/100 g DW) | TF (mg-QE/100 g DW) | RSA (mg-TE/100 g DW) | Macamide B (mg/100 g DW) |
Soxhlet extraction | n-Hexane | 0.24±0.02a | 0.04±0.00a | 0.32±0.02a | 101±2.64a | 7.80±0.61a | 25.8±0.69a | 2.78±0.12a |
1 N HCl-EtOH | 6.93±0.24d | 20.7±0.26c | 7.64±0.12c | 1,590±3.83d | 519±9.04c | 971±6.91d | 6.77±0.14b |
EtOH | 6.39±0.29c | 22.7±0.46d | 11.5±0.25d | 968±6.62c | 151±2.12b | 884±11.0c | 8.61±0.20c |
Water | 1.42±0.04b | 6.36±0.10b | 3.90±0.06b | 243±3.14b | 13.2±0.28a | 448±9.89b | NA |
Maceration extraction | n-Hexane | 0.12±0.01a | 0.03±0.00a | 0.35±0.02a | 43.2±1.80a | 12.3±0.20a | 23.4±0.74a | 4.55±0.16a |
50% n-Hexane | 1.55±0.12b | 3.51±0.12b | 2.83±0.09b | 109±3.53b | 30.5±1.08b | 52.3±1.62b | 7.17±0.17c |
1 N HCl-EtOH | 6.26±0.21d | 31.4±1.19d | 23.8±0.83f | 865±7.99d | 167±3.27f | 958±11.9f | 8.40±0.25d |
EtOH | 2.15±0.07c | 14.1±0.42c | 8.98±0.30c | 352±5.95c | 153±2.55e | 688±5.98e | 8.26±0.33d |
50% EtOH | 6.76±0.30e | 33.8±0.64e | 16.5±0.16e | 896±10.8e | 63.9±1.90d | 293±6.80c | 5.56±0.08b |
Water | 6.10±0.26d | 31.7±0.45d | 15.3±0.33d | 919±9.43f | 56.4±1.65c | 500±10.8d | NA |
NA, not available because of low concentration in the extracts.
Values in the same column indicated by different letters show significant differences (p<0.05).