Table 2. Effects of extraction parameters on the extraction yield in maceration extraction method using EtOH

Extraction condition			Extraction yield
Tempe-rature (°C)	Time (h)	Solvent-to-solid ratio	SP (g-BSAE/100 g DW)	TS (g-GE/100 g DW)	RS (g-GE/100 g DW)	TPC (mg-GAE/100 g DW)	TF (mg-QE/100 g DW)	RSA (mg-TE/100 g DW)	Macamide B (mg/100 g DW)
25	5	100:10	1.26±0.07^a	12.6±0.18^a	5.44±0.56^a	239±13.6^a	142±2.82^a	677±7.63^a	7.20±0.27^a
40			2.07±0.14^b	14.7±0.42^b	8.66±0.55^bc	345±5.59^b	165±2.89^b	692±7.68^b	8.42±0.33^b
50			2.15±0.06^b	15.6±0.31^c	9.33±0.59^cd	376±10.6^c	168±3.46^b	766±5.28^c	8.42±0.47^b
60			2.37±0.12^c	16.9±0.39^d	9.85±0.50^d	476±7.64^d	171±3.45^b	788±6.17^d	8.32±0.48^b
40	1.0	100:10	1.37±0.02^a	10.5±0.16^a	6.77±0.11^a	225±5.94^a	131±1.51^a	634±7.56^a	6.58±0.29^a
	2.5		1.81±0.05^b	14.0±0.26^b	8.67±0.12^b	330±10.4^b	145±2.53^b	662±12.1^b	7.80±0.32^b
	5.0		2.15±0.07^c	14.7±0.42^c	8.98±0.30^b	352±5.95^c	165±2.89^c	688±5.98^c	8.42±0.33^c
	7.5		2.21±0.14^c	16.3±0.37^d	9.76±0.44^c	375±9.02^d	166±2.80^c	707±10.3^cd	8.56±0.37^c
	10.0		2.26±0.21^c	16.3±0.33^d	9.62±0.35^c	394±10.1^e	176±2.71^d	725±14.9^e	8.67±0.25^c
40	5	100:1	3.72±0.67^c	23.9±0.52^e	14.0±0.23^e	NA	NA	NA	5.46±0.26^a
		100:5	2.56±0.10^b	19.7±0.39^d	12.1±0.47^d	405±10.3^d	155±1.32^d	769±17.2^d	8.15±0.18^c
		100:10	2.15±0.07^ab	14.7±0.42^c	8.98±0.30^c	352±5.95^c	166±4.73^c	688±5.98^c	8.42±0.33^c
		100:20	1.91±0.12^a	12.6±0.23^b	7.48±1.05^b	290±8.47^b	117±5.17^b	362±5.63^b	7.67±0.13^b
100:30	1.86±0.13^a	11.5±0.22^a	6.41±0.72^a	267±2.94^a	98.1±3.60^a	262±3.32^a	7.64±0.23^b

NA, not available because of low concentration in the extracts.
Values in the same column indicated by different letters show significant differences (p<0.05).