Table 7. Effect of various enzyme treatments on antibacterial activity of S. flavescens and S. chinensis 30% ethanol extract against Streptococcus mutans KCCM 40105

Enzyme concentration
Sample¹⁾		Clear zone on plate (mm/disk)
Sample¹⁾		Fun²⁾	Ter	Sec	Lac	Mal	Inv	Fla	Alc	Lip
SF	Control	9.87±0.60^3)ns4)	10.39±0.31^a6)	11.07±0.97^ab	10.90±1.14^ns	9.75±0.56^ab	10.33±0.38^a	10.66±0.76^ns	10.76±0.53^b	10.48±1.10^ns
	10 ppm	10.18±0.23	10.15±0.65^ab	9.84±0.41^b	10.95±1.13	9.82±0.97^ab	10.03±0.60^ab	10.31±0.74	10.52±0.46^b	9.82±0.74
	100 ppm	9.67±0.18	9.06±0.21^b	10.91±0.35^ab	9.85±0.94	8.72±0.35^b	9.58±0.39^a	10.73±0.41	10.40±0.27^b	11.25±1.08
	1,000 ppm	9.87±0.45	10.42±1.05^a	11.77±0.66^a	-	10.34±0.35^a	9.77±0.50^a	10.43±0.48	12.99±0.17^a	-
SC	Control	11.53±1.56	12.62±0.33^a	13.23±0.60^ns	13.49±2.08^ns	11.57±0.46^ns	12.81±3.22^ns	12.66±1.57^ns	13.02±3.75^ns	14.33±1.82^ns
	10 ppm	12.36±0.45	11.80±1.55^a	12.10±3.15	13.11±2.72	12.84±0.25	12.21±1.52	13.88±0.39	11.73±2.76	12.78±0.86
	100 ppm	10.58±1.65	8.83±0.04^b	11.19±2.22	11.36±2.65	10.94±1.34	13.50±2.51	11.51±0.64	12.16±3.94	12.07±1.16
	1,000 ppm	-⁵⁾	-	10.23±1.40	-	10.72±0.87	11.95±0.71	12.31±1.83	10.63±2.31	-
Enzyme reaction temperature
SF	Control	11.01±0.39^3)a6)	9.48±1.02^ns	10.67±0.49^a	9.69±1.18^ns	8.71±0.29^ab	9.04±0.74^bc	10.70±0.79^ab	10.33±0.64^ns	9.78±1.10^ns
	20° C	9.67±0.79^ab	9.33±0.07	9.89±0.53^b	9.30±0.42	8.90±0.34^ab	10.09±0.52^a	11.55±0.27^a	10.29±0.52	9.61±1.17
	25° C	10.15±1.22^ab	9.20±0.39	8.97±0.59^cd	9.52±1.00	8.95±0.47^ab	9.21±0.32^abc	10.90±0.35^ab	10.02±0.66	9.77±0.74
	30° C	9.16±0.56^b	9.00±0.20	9.41±0.26^bc	10.52±0.77	8.33±0.05^b	8.49±0.10^c	9.71±1.24^b	10.22±0.31	9.72±1.39
	35° C	9.89±0.70^ab	9.74±0.24	8.67±0.06^cd	9.94±1.39	9.26±0.60^a	9.77±0.32^ab	11.15±0.55^a	9.51±1.24	9.80±0.94
	40° C	9.79±0.93^ab	9.12±0.42	8.55±0.19^d	9.70±1.07	9.12±0.53^ab	9.37±0.55^abc	10.78±0.50^ab	9.75±1.08	10.41±0.90
SC	Control	11.97±2.72^ns4)	11.34±0.37^a	12.16±3.90^ns	14.27±2.62^ns	10.14±0.68^ab	13.40±2.49^ns	11.73±1.83^a	9.86±1.66^ns	9.85±1.47^ns
	20° C	10.33±2.83	11.07±0.54^a	10.37±1.74	11.13±2.66	11.17±0.82^a	12.39±1.80	12.59±2.52^a	8.93±1.25	10.45±1.64
	25° C	10.84±0.21	10.19±0.26^ab	9.37±1.02	12.46±1.25	9.87±0.65^ab	12.76±0.92	11.56±3.21^a	9.28±0.86	10.44±1.84
	30° C	10.77±2.23	10.21±0.41^ab	9.81±1.65	10.99±2.40	8.93±0.83b	11.95±2.11	12.70±2.88^ab	11.39±2.84	10.82±2.72
	35° C	10.20±2.75	10.77±0.70^ab	9.12±0.56	10.39±1.24	11.37±0.99^a	12.75±1.10	13.66±2.59^a	9.03±0.66	10.76±1.81
	40° C	8.87±0.36	9.52±1.09^b	9.88±1.68	11.04±2.52	10.69±0.79^a	13.35±0.58	12.44±2.48^a	11.27±3.39	11.61±3.31

SF, Sophorae flavescens; SC, Schizandra chinensis.
Fun, fungamyl; Ter, termamyl; Sec, secura; Lac, lactozym; Mal, maltoginase; Inv, invertase; Fla, flavourzyme; Alc, alcalase; Lip, lipozyme.
All values are mean±SD (n=3).
Means with letters in the same column are not significantly different at p<0.05 by Duncan’s multiple range test.
Not detected.
Means with different superscript letters in the same column are significantly different at p<0.05 by Duncan’s multiple range test. a>b>c.