Table 1. LOD, LOQ and linearity for vitamin B6 and B12 analyses

Analytes Analytical methods Calibration curve1) R2 LOD2) (μg/100 g) LOQ3) (μg/100 g)
Vitamin B6 Pyridoxine HPLC-FLD y=90.4607x-0.0559 0.9999 0.008±0.006 0.022±0.017
Pyridoxal y=73.3855x-0.0069 1.0000 0.007±0.002 0.015±0.006
Pyridoxamine y=77.9059x-0.0192 0.9999 0.007±0.003 0.018±0.007
Vitamin B12 Cyanocobalamin Immunoaffinity-HPLC-PDA y=2,007.7232x-0.3788 1.0000 0.005±0.004 0.012±0.011
y=ax+b: y and x indicate peak area (mAU) and concentration (μg/100 g), respectively.
Limit of detection.
Limit of quantification.