Table 1. LOD, LOQ and linearity for vitamin B6 and B12 analyses
Analytes | | Analytical methods | Calibration curve1) | R2 | LOD2) (μg/100 g) | LOQ3) (μg/100 g) |
Vitamin B6 | Pyridoxine | HPLC-FLD | y=90.4607x-0.0559 | 0.9999 | 0.008±0.006 | 0.022±0.017 |
Pyridoxal | | y=73.3855x-0.0069 | 1.0000 | 0.007±0.002 | 0.015±0.006 |
Pyridoxamine | | y=77.9059x-0.0192 | 0.9999 | 0.007±0.003 | 0.018±0.007 |
Vitamin B12 | Cyanocobalamin | Immunoaffinity-HPLC-PDA | y=2,007.7232x-0.3788 | 1.0000 | 0.005±0.004 | 0.012±0.011 |
y=ax+b: y and x indicate peak area (mAU) and concentration (μg/100 g), respectively.
Limit of detection.
Limit of quantification.