Table 1. The gradient condition of mobile phase for β-carotene analysis by HPLC

Time (min)	Solvent A	Solvent B
	Methanol/Buthanol/Water (v/v/v)
	60/10/13	89.5/10/0.5
0	75	25
8	75	25
50	90	10
55	90	10
57	75	25
65	75	25