Table 2. Range of coded variables at different levels and results for biomass production by central composite design

Factor		Symbol (unit)	Coded values
			-3	-1		0	+1		+3
Glucose		X1 (g/L)	0	10		15	20		30
Sucrose		X2 (g/L)	0	10		15	20		30
Yeast extract		X3 (g/L)	10	20		25	30		40
Run	X1		X2		X3			R11)
1	+1		+1		-1			4.20e+08
2	0		0		+3			1.11e+09
3	0		0		-1			2.10e+08
4	-1		-1		+1			6.75e+08
5	+1		+1		+1			1.41e+09
6	-1		-1		-1			1.00e+09
7	0		+3		0			7.30e+08
8	+1		-1		-1			5.35e+08
9	0		0		0			7.85e+08
10	+1		-1		+1			7.10e+08
11	-1		+1		-1			3.60e+08
12	0		0		0			7.50e+08
13	+3		0		0			5.65e+08
14	-1		+1		+1			7.85e+08
15	0		-3		0			7.85e+08
16	0		0		0			7.75e+08
17	-3		0		0			3.70e+08

R1 viable cell count of A. pasteurianus SRCM101388.