Analysis condition | UPLC-FLD | HPLC-FLD |
---|---|---|
Instrument | WATERS ACQUITY | HITACHI Chromaster |
Column | WATERS Xselect CSH C18 | HITACHI LaChrom C18-AQ |
Mobile phase (ACN : MeOH : Water) | 15 : 20 : 65 (v/v/v) | 10 : 30 : 60 (v/v/v) |
Flow rate | 0.2 mL/min | 1.0 mL/min |
Run time | 10 min | 20 min |
Column temperature | 40°C | 40°C |
Injection volume | 10 | 10 |
FL detector wavelength | EX 360 nm, Em 440 nm | EX 365 nm, Em 450 nm |