Table 1. UPLC and HPLC conditions for the aflatoxin analysis

Analysis condition UPLC-FLD HPLC-FLD
Instrument WATERS ACQUITY UPLC H Class HITACHI Chromaster CM5000 Series
Column WATERS Xselect CSH C18 (2.5 μm, 2.1 mm I.D. × 100 mm) HITACHI LaChrom C18-AQ (3 μm, 4.6 mm I.D. × 150 mm)
Mobile phase (ACN : MeOH : Water) 15 : 20 : 65 (v/v/v) 10 : 30 : 60 (v/v/v)
Flow rate 0.2 mL/min 1.0 mL/min
Run time 10 min 20 min
Column temperature 40°C 40°C
Injection volume 10 μL 10 μL
FL detector wavelength EX 360 nm, Em 440 nm EX 365 nm, Em 450 nm