Table 1. HPLC operating conditions for vitamin B1 analysis
Items
Vitamin B
1
Column temp.
20°C
Detector
UV 270.4 nm
Injection volumn
20 μL
Flow rate
1.0 mL/min
Mobile phase
(A) 5 mM sodium 1-hexanesulfonate 80: methanol 20 (acetic acid 0.75%, triethylamine 0.02%)
(B) Methanol
Gradient condition
0 min: A 100%
19 min: A 100%
20 min: A 100%
41 min: A 100%
42 min: A 45% + B 55%
49 min: A 45% + B 55%
50 min: A 100%