Table 3. Validation results for vitamin B₁ and B₃

Component		Calibration curve	Linearity1)	LOD2) (μg/mL)	LOQ3) (μg/mL)
Vitamin B1	Thiamin	y=46.787x+0.2802	1.0000	0.004	0.012
Vitamin B3	Nicotinic acid	y=335.92x+0.4314	1.0000	0.005	0.015
	Nicotinamide	y=88.529x+2.1255	1.0000	0.002	0.006

Calibration range: 0.1-10 μg/mL, 7-point.
LOD, limit of detection.
LOQ, limit of quantitation.