Table 1. Comparison of real-time polymerase chain reaction1), loop-mediated isothermal amplification2), and agar plate method for detection of Salmonella spp. in ready-to-eat3) food (n=3)
| RT-PCR | LAMP | Agar plate |
SureTect⢠kit | PowerChek⢠kit | MDS | XLD |
Inoculation level (CFU/mL) | Inoculation level (CFU/mL) | Inoculation level (CFU/mL) | Inoculation level (CFU/mL) |
0-10 | 10-50 | 50-100 | 100- | 0-10 | 10-50 | 50-100 | 100- | 0-10 | 10-50 | 50-100 | 100- | 0-10 | 10-50 | 50-100 | 100- |
Sandwich | 34)/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
Sausage | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
Hamburger | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
Kimbap | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
Bacon | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
Salad | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
RT-PCR, real-time polymerase chain reaction.
LAMP, loop-mediated isothermal amplification.
RTE, ready-to-eat.
Means the number of detected samples among three iterations.