Table 2. Threshold cycle (Ct) values under different annealing temperatures and times

Primer name	Sample	58°C		59°C		60°C
		15 s	25 s	15 s	25 s	15 s	25 s
Oncorhynchus keta	Negative	-1)	-	-	-	-	-
	Oncorhynchus keta	19.32	19.04	19.27	19.15	19.78	19.12
	Oncorhynchus mykiss	23.02	23.18	23.07	23.30	23.95	24.11
Oncorhynchus mykiss	Negative	-2)	-	-	-	-	-
	Oncorhynchus keta	19.76	19.85	20.22	20.42	20.70	21.01
	Oncorhynchus mykiss	15.48	15.61	15.43	15.56	15.17	15.35

-, Ct value of 24 or more.
-, Ct value of 20 or more.