Table 6. Threshold cycle (Ct) values of calibrator in ultra-fast quantitative real-time PCR for Oncorhynchus keta and Oncorhynchus mykiss

Species	Test level (%)	Ct value			Mean	SD1)
		Repeat 1	Repeat 2	Repeat 3
Oncorhynchus keta	100	19.10	19.80	19.34	19.41	0.36
	70	20.16	20.02	20.92	20.37	0.48
	50	21.01	20.97	20.15	20.71	0.49
	30	21.12	21.95	22.02	21.70	0.50
	10	22.81	22.92	23.02	22.92	0.11
Oncorhynchus mykiss	100	15.52	15.74	15.42	15.56	0.16
	70	17.10	16.42	16.28	16.60	0.44
	50	17.11	17.36	17.20	17.22	0.13
	30	18.07	18.26	18.10	18.14	0.10
	10	19.10	19.28	19.01	19.13	0.14

SD, standard deviation.