Table 7. Calibration results in ultra-fast quantitative real-time PCR for Oncorhynchus keta and Oncorhynchus mykiss

Species	Parameter	Repeat 1	Repeat 2	Repeat 3	Mean
Oncorhynchus keta	Slope	−3.4	−3.3	−3.5	−3.4
	PCR efficiency (%)	96.53	93.67	101.10	97.10
	Linearity (R2)	0.99	0.99	0.99	0.99
Oncorhynchus mykiss	Slope	−3.23	−3.5	−3.5	−3.4
	PCR efficiency (%)	103.96	92.14	91.21	95.77
	Linearity (R2)	0.99	0.99	0.99	0.99