Table 4. The results of sequencing and diversity analysis in makjang between outdoors and indoors locations after 90 days of fermentation
| Groups | Sample | Total reads | High-quality reads | ASVs2) | Chao13) | Shannon4) | Simpson5) | Good’s coverage6) |
| A1) | B | A | B | A | B | A | B | A | B | A | B | A | B |
| Outoors | Control7) | 111,932 | 129,540 | 109,800 | 127,923 | 43.00 | 21.00 | 43.00 | 21.00 | 3.40 | 2.21 | 0.87 | 0.66 | 1.00 | 1.00 |
| S3 | 125,659 | 124,441 | 123,942 | 122,761 | 55.00 | 28.00 | 55.17 | 28.00 | 3.52 | 2.47 | 0.88 | 0.75 | 1.00 | 1.00 |
| M | 81,400 | 145,483 | 80,056 | 143,890 | 41.00 | 28.00 | 41.00 | 28.00 | 3.36 | 2.81 | 0.85 | 0.79 | 1.00 | 1.00 |
| Indoors | Control | 120,293 | - | 118,690 | - | 41.00 | - | 41.33 | - | 3.42 | - | 0.87 | - | 1.00 | - |
| S3 | 94,830 | 152,880 | 93,325 | 151,086 | 64.00 | 26.00 | 65.20 | 26.00 | 4.00 | 1.27 | 0.92 | 0.34 | 1.00 | 1.00 |
| M | 101,661 | 169,079 | 100,197 | 167,131 | 43.00 | 24.00 | 43.00 | 24.00 | 3.47 | 0.61 | 0.87 | 0.14 | 1.00 | 1.00 |
Diversity indices of are compared between the bacterial (A) and fungal (B) communities in makjang distinguished into outdoors and indoors.
ASVs (amplicon sequence variants) are the inferred unique sequences after correcting for sequencing and PCR errors.
Chao1 was calculated to estimate the richness based on the ASVs.
Shannon was calculated to estimate the diversity based on the ASVs.
Simpson is the inferred value of the diversity and evenness of species based on the ASVs.
Good’s coverage estimated the proportion of species represented in the sampled data based on the ASVs.
Control, no starter during the manufacture of makjang ; S3, manufactured using only E. faecium N78-11; M, manufactured using all of S1, S2, and S3 strains.