| GC-MS/MS | Column | 5MS (30 m, 0.25 mm×0.25 μm) |
| Column oven | 60°C → 20°C/min → 180°C → 5°C/min → 300°C → 20°C/min → 310°C (2 min) |
| Injection temperature | 280°C |
| Injection volume | 1 μL |
| Carrier gas | He, 1.2 mL/min |
| Collision gas | Argon |
| Ion source temperature | 250°C |
| Interface temperature | 300°C |
| LC-MS/MS | Column | CAPCELL CORE C18 (2.7 μm, 2.1 mm×150 mm) |
| Column oven | 40°C |
| Mobile phase | A: 0.1% formic acid, 5 mM ammonium formate in waterB: 0.1% formic acid, 5 mM ammonium formate in methanol |
| Injection volume | 2 μL |
| Flow rate | 0.2 mL/min |
| Gradient program | Time (min) | Mobile phase |
| A (%) | B (%) |
| Initial | 95 | 5 |
| 1.0 | 95 | 5 |
| 3.0 | 40 | 60 |
| 13.0 | 0 | 100 |
| 18.0 | 0 | 100 |
| 18.1 | 95 | 5 |