Table 1. HPLC condition for analyzing phenolic compounds

Items Conditions
Instrument Arc HPLC system(Waters, Milford, MA, USA)
Column Zorbax eclipse XDB-C18 4.6×250 mm, 5 μm(Agilent technologies, SantaClara, CA, USA)
Detector PDA Detector(Ex: 210 nm, Em: 280 nm)(2998, Waters, Milford, MA, USA)
Mobile phase A: 0.1% formic acid in 10% AcetonitrileB: 0.1% formic acid in 90% Acetonitrile
Gradient 0-2 min A : B = 100 : 0
2-8 min A : B = 100 : 0
8-35 min A : B = 90 : 10
35-40 min A : B = 45 : 55
40-45 min A : B = 50 : 50
45 min A : B = 100 : 0
Flow rate 1.0 mL/min
Column temp 35°C
Injection volume 10 μL