Specification | Conditions |
---|---|
HPLC | Agilent 1260 Infinity II |
Column | Supelcogel C-610H (300 mm×7.8 mm, 9 μm) |
Osaka C18(150 mm×2.1 mm, 2.7 μm) | |
Mobile phase | 0.1 M Phosphoric acid (H3PO4) |
Analysis time | 45 min |
Flow rate | 0.5 mL/min |
Column temperature | 30°C |
Injection volume | 20 μL |
Detector | UV 210 nm |