Table 7. Repeatability and reproducibility of different extraction methods for biotin analysis of infant formula

Sample Extraction method Biotin (μg/100 g)1)
Repeatability2) Reproducibility3)
Mean±SD RSD (%) Mean±SD RSD (%)
In house quality control Autoclave Non-treated 31.924±0.572 1.793 32.265±0.180 0.556
Pepsin-assisted 31.957±1.029 3.222 31.394±1.161 3.698
All values are mean±SD (n=3).
Repeatability refers to the results of 5 independent determinations carried out on a sample by analysing 3 replicates of the sample on the same day.
Reproducibility refers to the results of 5 independent determinations carried out on a sample by analyzing 3 replicates of the sample at each day for 5 days.