Table 1. HPLC gradient conditions

Time (min)	Mobile phase-A (%)	Mobile phase-B (%)	Flow rate (mL/min)
0.0	99.0	1.0	0.7
7.0	80.0	20.0	0.7
14.0	0.0	100.0	0.7
17.0	0.0	100.0	0.7
18.1	99.0	1.0	0.7
21.0	99.0	1.0	0.7
25.0	100.0	0.0	0.7